The Jamaican quartet of Tyquendo Tracey, Julian Forte, Micheal Campbell and Bolt led the eight-team second heat from beginning to the end.
And Bolt relished the adulation at the 60,000-capacity Olympic Stadium.
"Even if he was just closing on the guys ahead - he wanted to give this crowd a show so they can say "I saw Usain Bolt run down the finishing straight".
For the best part of the past decade, though, Bolt has been 100 percent reliable in getting relay gold for the island nation.
The sprint legend, who had won the 100m, 200m and 4x100m Gold at the same venue during the 2012 Olympics, might not have had a flawless end to his career, but his records over the years will definitely make him a once-in-a-lifetime athlete. And the result in the relay is no longer a foregone conclusion.
Great Britain won the gold in 37.47 seconds while United States (37.52) and Japan (388.04) took the silver and bronze respectively.
"It would be pretty awesome", he said of his bid to win a fourth world 5,000m gold following victories in 2011, 2013 and 2015.
The U.S. women, meanwhile, had a better night.
GETTYUsain Bolt refused the offer of a wheelchair despite collapsing with an injury
Bolt's charisma, charm and character have made him one of the most recognisable and marketable people on the planet.
Still, Bolt was supportive of his young teammates.
"They were holding us too long in the call room", Yohan Blake told the media.
Nevertheless, Twitter was filled with praises and cheerful messages for Bolt, as they hailed him for being a legend on track.
It was yet another awesome upset in a championship of so many. They also advanced to the final. Olympic champion Thomas Roehler of Germany finished fourth.
The Netherlands, with 200-meter champion Dafne Schippers running the second leg, qualified on time. "My training will definitely be different and I'll be ready to run the 100 and 200 in the next world championships for sure".
It was Jamaica's first loss in a Men's 4x100m relay at the World Championships since 2007.
Usain Bolt was the overwhelming favourite to win the 100m race as he planned to retire after this tournament but things didn't quite work in his favour as he finished in third place behind Christian Coleman in second place and Jason Gatlin in the first place.
England ease past Italy in Women's Rugby World Cup
A few minutes later, Alex Matthews touched down under the posts to leave Scaratt with an easy conversion to make it 12-0. England have made 10 changes for their World Cup match against Italy in Dublin with Emily Scarratt leading the team.
Tom Cruise appears injured after slamming into a wall while filming a stunt for the upcoming movie " Mission: Impossible 6 ". Cruise explained , "I was hit by a tiny particle from the runway and I literally thought it broke my ribs".
Olivier Giroud has been linked with a move away from Arsenal with the arrival of fellow Frenchman Alexandre Lacazette from Lyon. Arsene Wenger has told Arsenal fans that Olivier Giroud is staying at the club this season.
The fact sheet tells people how to prepare for an attack, and what to do if, heaven forbid, an attack actually occurred. Guam is home to a U.S. air base, a Navy installation, a Coast Guard group and roughly 6,000 U.S. military personnel.
The former Juventus man had a slow start to the 2016-17 season, after scoring just 5 goals in as many as 30 matches for the club. Manchester United won the EFL Cup and the Europa League last season to mask over their Premier League disappointment.
According to the Indian law, despite the will of the donor final approval over the donation is subject to the deceased's family. Organ Donation Day is celebrated on 13th of August every year by the people in India.
This buyback authorization authorizes the transportation company to repurchase shares of its stock through open market purchases. Finally, Carderock Capital Management Inc. raised its position in Canadian National Railway by 0.6% in the first quarter.
Le Losc s'attend par ailleurs à être attaqué par des formations d'outre-Manche lors de la dernière semaine d'août. Marcelo Bielsa en a pour le moment fait son titulaire à la pointe de l'attaque lilloise.
Guliyev surprend Van Niekerk sur 200 m — Mondiaux
Les deux hommes ont été séparés d'un millième de seconde, ce qui n'est pas beaucoup, précisons pour les non-spécialistes. La perf Isaac Makwala? Makwala prétend qu'il aurait pu gagner et il était à coup sûr le seul danger pour Van Niekerk.